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991.
992.
Zhen-Xia Sha Shi-Chao Xing Chang-Wei Shao Yong-Sheng Tian Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(6):1799-1801
Ladyfish (Elops saurus Linnaeus) is an economically important marine fish species. 76 microsatellite loci were isolated from an enriched genomic library
of Elops saurus. Twelve of these markers were polymorphic in a test population with alleles per locus ranging from three to nine. The number
of observed, expected heterozygosity and polymorphism information content (PIC) per locus in 20 individuals ranged from 0.2000
to 1.0000, 0.1897–0.8846, 0.1769–0.8476, respectively. One markers significantly deviated from Hardy-Weinberg equilibrium
after Bonferroni correction analysis and there was no significant linkage disequilibrium found between pairs of markers. As
a result, 12 microsatellite markers probably should provide sufficient level of genetic diversity to investigate the fine-scale
population structure, stock management and enhancement, genetic linkage map construction and molecular marker-assisted breeding
in Elops saurus Linnaeus. 相似文献
993.
Gui-Dong Miao Chang-Wei Shao Xiao-Lin Liao Hong-Yu Ma Yong-Sheng Tian Song-Lin Chen 《Conservation Genetics》2009,10(3):701-703
Barfin flounder (Verasper moseri) is a rare fish species in the world. Here, we reported 10 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of barfin flounder (Verasper moseri). The number of alleles, observed, and expected heterozygosity per locus in a test population ranged from 2 to 6, from 0.3333
to 1.0000, and from 0.4866 to 0.7774, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. Cross-species amplification
of these microsatellite loci in additional five fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in Verasper moseri.
Gui-Dong Miao and Chang-Wei Shao Contributed equally to this work. 相似文献
994.
Changwei Shao Songlin Chen Genbo Xu Xiaolin Liao Yongsheng Tian 《Conservation Genetics》2009,10(3):623-625
Chinese sea perch (Lateolabrax maculates) is one of the most important commercial species of mariculture in China. In this study, we constructed a repeat-enriched
genomic DNA library of L. maculates. Eighteen dinucleotide microsatellite markers were characterized by genotyping 32 samples. The number of alleles ranged from
three to nine, and the observed and expected heterozygosities ranged from 0.4516 to 1.0000 and from 0.4045 to 0.8676, respectively.
Significant deviations from Hardy–Weinberg expectations were detected at four loci and linkage disequilibrium between two
loci was significant after applying Bonferroni correction. The 18 highly polymorphic microsatellite markers should provide
sufficient level of genetic diversity to investigate the population structure and evaluate the breeding strategy in L. maculates. 相似文献
995.
996.
Tianjun Xu Changwei Shao Xiaolin Liao Xiangshan Ji Songlin Chen 《Conservation Genetics》2009,10(3):527-529
From a (GT)13-enriched genomic library of Oplegnathus fasciatus, 14 polymorphic microsatellite were isolated and characterized in a test population with alleles ranging from two to nine,
the observed and expected heterozygosities from 0.0000 to 1.0000, and from 0.1726 to 0.8507, respectively. Five loci deviated
from the Hardy–Weinberg equilibrium, and linkage disequilibrium between two loci was significant. Two loci were also polymorphic
in Pagrosomus major assessed for cross-species amplification. These polymorphic microsatellites will be useful for genetic diversity analysis
and molecule-assisted breeding for O. fasciatus.
Changwei Shao contributed equally. 相似文献
997.
Hao Ding Changwei Shao Xiaolin Liao Genbo Xu Xiangshan Ji Songlin Chen 《Conservation Genetics》2009,10(3):611-614
In the present study, 10 polymorphic microsatellite DNA loci from Atlantic halibut (Hippoglossus hippoglossus) were isolated and characterized. The number of alleles for these loci ranged from 2 to 4 in tested 24 individuals. Observed
and expected heterozygosities per locus varied from 0.21 to 0.70 and from 0.31 to 0.65, respectively. Most of these 10 microsatellite
loci were successfully amplified and showed polymorphic in five related species. These loci will be useful for the assessment
of genetic diversity and population structure of Atlantic halibut.
H. Ding and C. Shao contributed equally to this work. 相似文献
998.
999.
1000.